The new software contains four modules: Polo JR, PoloMulti, PoloMixture, and Optidose. The programs were developed from scratch using the original statistical models. The code is more streamlined and can be run on personal computers supporting Windows 7, 8, 10, and MacOS operating systems. The graphs have been updated, and laborious steps for creating individual parameter files for PoloMix have been removed.
PoloJR© computes probit (or logit) quantal regression analyses and conducts automatic tests of the hypotheses of parallelism and equality. Several features enhance the program’s use in toxicological and pharmaceutical testing. First, lethal dose ratios are estimated for statistical comparison of each preparation with a standard. Second, standardized residuals are calculated. Third, the residuals are displayed in plots for identification of sources of lack-of-fit to the probit or logit model. Fourth, a fitted response curve for each preparation can be plotted. Data can now be entered directly into the program rather than loading it as a text file. The end product can also be exported to excel for individual graphing needs.
PoloMulti© does the work of PoloJR but extends it to binary response models with multiple explanatory variables. Such models are rather common in bioassays with chemicals such as pesticides and pharmaceuticals. They provide an efficient means to use a single experiment to test the significance of more than one variable (such as dose) on response.
PoloMixture© uses a chi-square statistic to test the hypothesis of independent joint action. This test is done by obtaining an estimate for the probability of mortality p for several dose or concentration levels of the two components and then comparing the estimate of p with the observed proportion killed at the corresponding dose levels. The three contributions to p are estimated separately. First, p0 is calculated as the proportional mortality observed in the control group. Next, p1 and p2 are estimated from bioassays of component 1 and component 2, with data analyzed with PoloJR. Finally, the chi-square test statistic is calculated. We updated this module to remove parameter files, include a chi-square table for hypothesis testing, and added graphing potential.
OptiDose© is a program designed specifically for the study of dose placement and sample size for any dose that would cause mortality greater than zero and less then 100%. OptiDose calculates the length of the confidence interval for µ0, the dose level that produces a certain probability of response P0, where 0<P0<1. The program is written to assist in the selection of the optimal doses to use in a bioassay that involves binary (e.g., dead vs. alive) response. The results of the bioassay can then be analyzed by PoloPlus.
These software programs are discussed in the book, Bioassays with Arthropods, Second Edition, by Jacqueline Robertson, Robert M. Russell, Haiganoush Preisler, and N. Eugene Savin. (Taylor & Francis CRC Press, 2007).
Moneen Jones on ResearchGate
All four programs are contained on a single electronic file. An HTML help file is built in for easy word search and definitions. The program is available for Windows and MacIntosh operating systems.
$225 per licensed download of all four modules. License is renewal on a yearly basis. We accept PayPal and all major credit and debit cards.
If during your year of license ownership, a new module (besides the first four basic) becomes available. You will have the option to upgrading your software immediately for $50 or wait until your license is going to expire and purchase the new version then.